World Journal of Agricultural Sciences 1 (1): 62-64, 2005
Compatibility of Phyton with Common Fungicides and Their Role on the Yield of Safed Musli
1Department of Studies in Applied Botany, Seed Pathology and Biotechnology,
University of Mysore, Manasagangothri, Mysore - 570006, India
2Agricultural Consultancy Research and Educational Services,
Abstract: In order to know the compatibility of Phyton (a Phytotonic) with routine fungicides like Captan and
GLSTIN (Carbendazim), for the preliminary management of Fusarium solani, a causal organism of tuber-rot of
safed musli, the present study was carried out. Among the fungicides, though GLSTIN stood superior, its effect
was found inferior to that of Phyton alone. However, the combination of Phyton with GLSTIN @ 0.3% each was
proved to be the best in reducing the growth of the fungus. The same combination was also proved its
efficiency in the enhancement of the yield of the crop. Key words: Tuber-rot phytotonic-phyton fungicide treatments crop yield INTRODUCTION MATERIALS AND METHODS
Safed musli (Chlorophytum borivilianum), a herb
Healthy tubers of Chlorophytum borivilianum
belongs to family Liliaceae. It was originally grown in
Santapau and Fernandes were collected from the
thick forests of India[1]. About 300 species are distributed
agricultural field at Mysore, Karnataka State in India and
throughout the tropical and subtropical parts of the world.
were kept in plastic trays under room conditions for
Tropical and subtropical zones of Africa are the probable
further use. These tubers were further washed and used
centers of origin of the genus[2]. In India, it is considered
for soaking treatment in 0.3% solutions of fungicides like
as a valuable medicinal herb, whereas in other parts of the
Captan, GLSTIN and Phyton. During treatment 25 tubers
world it is being used as ornamental plant. The roots
in each set were separately soaked in 0.3% Captan,
(tubers) are rich in alkaloids, vitamins, minerals, proteins,
GLSTIN, Phyton and in a combined solution of 0.3%
carbohydrates, saponins, polysaccharides and steroids
Phyton and 0.3% GLSTIN for 18 h at 28±2°C. After
of various therapeutic values as total rejuvenator,
soaking treatment, the tubers were sown in the pots
antioxidant and Immuno modulator[3]. It is being used
contained sand, soil and farmyard manure in the ratio of
as an anti arthritic and anticancer drug. Because of
2:2:1. The pots were maintained at green-house conditions
its aphrodisial properties, it is mainly identified as
for a period of 9 months and then the tubers were
‘Herbal viagra’. Safed musli is widely distributed in India
harvested. On harvest, the average biomass of the tubers
mainly in Southern Rajasthan, Western Madhya Pradesh,
of each treatment was measured and the data were
North Gujarat and few parts of Karnataka. But, continuous
tabulated. The tubers without treatment with chemicals,
exploration has decreased its frequency, distribution and
maintained under similar conditions were served as
the quality. This species has low rate of regeneration and
corresponding control. In the other set, tuber-rot causing
prone to attack by many diseases. Among them tuber-rot
fungus, Fusarium solani was isolated from the rotted
and crown-rot are more prevalent in Agricultural fields at
tubers of crown-rot showing plants and maintained in the
Mysore district of Karnataka State in India. Since the
laboratory for further studies. In order to find out an
cultivation expenses are very high, it is mandatory to get
effective treatment to manage the incidence of F. solani
disease a free product. Hence, in the present study to
in tubers and to improve its yield, in the present study the
overcome the problem of tuber-rot and the crown-rot,
above mentioned chemicals in the same concentrations
attempts have been made using fungicides for tuber
were amended to Potato Dextrose Agar medium. Then,
treatment as well as foliar spray under green-house
uniform culture discs of 5 mm diameter were obtained from
the 8-day-old actively growing colonies of F. solani and
Corresponding Author: Dr. S. Lokesh, Department of Studies in Applied Botany, Seed Pathology and Biotechnology,
University of Mysore, Manasagangothri, Mysore - 570006, India
World J. Agric. Sci., 1 (1): 62-64, 2005
Fig. 1: Effect of different fungicides and Phyton - a Phytotonic on the mycelial growth of Fusarium solani
Mean length of tubers at harvest (cm)Mean total number of tubers/plant
Fig. 2: Role of Phyton and some fungicides on the yield of Safed Musli
were individually plated on the poisoned medium and
which was varied with respect to different fungicides
were incubated for a period of one week under 12/12 h
and Phyton. Comparatively, the growth of the fungus
alternate cycles of near ultraviolet light and darkness at
was observed to be more in Captan treated plates.
22±2°C according to the procedures of ISTA[4]. On 8th
Phyton and GLSTIN @ 0.3% indicated the reduced
day of incubation the colony diameter of the fungus was
growth of the fungus. This combination was found to be
recorded based on the average of three replicates. While
the best compared to the control and the fungicide
measuring, four different sectors of each colony were
amended medium, which was resulted in the reduction of
considered to get consistent data. In this case also the
the colony diameter by more than two folds.
cultures grown on PDA plates unamended with chemicals
Data with respect to mean length of the tubers, total
number of tubers/plant and mean biomass of the
individual tubers at harvest are represented in Fig. 2. RESULTS AND DISCUSSION
Compared to control all the treatments enhanced the total
yield of the tubers. Among the treatments, combination of
Data indicated in Fig. 1 provides the details of the
Phyton with GLSTIN was found to be promising in
colony growth of the causal organism Fusarium solani
enhancing the yield of the crop to a maximum extent. World J. Agric. Sci., 1 (1): 62-64, 2005
Though Phyton alone was putative, it was much
ACKNOWLEDGEMENT
beneficial in combination with GLSTIN. Phyton is known
to enrich with potash and phosphorus, which might have
Sincere thanks are due to Prof. Prakash, H.S.
facilitated the healthy growth of the plant, thereby
Chairman, Department of Studies in Applied Botany,
enhanced the tuber development, ultimately the yield.
Seed Pathology and Biotechnology, University of
Generally potash and phosphorus are known to play an
Mysore, Manasagangothri, Mysore - 570006, India, for
important role in the enrichment of leaves with high
co-operation throughout the work in the department.
chlorophyll content. In contrast high nitrogenous supply
make the plants highly vulnerable to the fungal diseases. REFERENCES
In the soil as such the availability of phosphorus is
meager due to acidic condition of soil. Hence, Phyton
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Karyomorphology of four diploid species of
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Chlorophytum. Nucleus, 15: 39-45.
resulted in the high yield of the tubers. The combination
2. Geetha, K. and S. Maiti, 2002. Biodiversity
of Phyton with GLSTIN also favoured the plants in which
Chlorophytum borivilianum, Santapau and
GLSTIN safeguarded the plants systematically against the
Fernandes. International Plant Genetic Resource
tuber-rot causing organism F. solani. So, rather than the
individual chemical treatment, combination proved their
3. Anonymous, 2001. Medicinal plants more on safed
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4. Anonymous, 1996. International Rules of Seed
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The efficacy of the chemicals is always dose
thiabendazole and other fungicides. Crop Protection,
dependent, which also varies with respect of method of
treatment and duration of treatment. In other ways, the
surface texture of the host tissues also plays an important
role in the absorption of the chemical compounds. In this
regard it is necessary to fetch more information for the
betterment of a crop yield against the tuber-rot fungus.
GYÓGYSZER INTERAKCIÓ A MAGZAT FEJL Ő DÉSI ZAVARAINAK LÉTREJÖTTÉBEN. (A TARDYL® NEUROTOXIKUS HATÁSA EMBERI MAGZATBAN) Petik Dóra1, Czeizel Endre2, Szili Réka3, Bódis József3-4, Kriszbacher Ildikó3 1Fővárosi Szent István és Szent László Kórház, Szülészeti - Nőgyógyászati Osztály, Budapest 2Genetikai Ártalmak Társadalmi Megelőzése Alapítvány, Budap