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L. mono Differential Agar Base
L. mono Differential Agar Base has been recommended for the selective and differential isolation of Listeriamonocytogenes .
Composition**
Ingredients
Gms / Litre
**Formula adjusted, standardized to suit performance parameters Directions
Suspend 36.02 grams in 460 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving
at15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Aseptically add sterile contents of 1 vial of L. mono Enrichment Supplement I (FD214) and sterile rehydrated contents of L .mono Selective Supplement I (FD212), L .mono Selective Supplement II (FD213). Mix well and pour into sterile Petri plates.
Warning : Lithium chloride is harmful. Avoid bodily contact and inhalation of vapours. On contact with skin wash with plenty Principle And Interpretation
Listeria monocytogenes is a gram-positive foodborne human pathogen responsible for serious infections in pregnant women that may ultimately result in abortion, stillbirth, birth of a child with neonatal listeriosis and meningitis or primary bacteremia in adults and juveniles. The pathogenicity of Listeria ivanovii for humans is uncertain. Since L. monocytogenes and L. innocua have similar biochemical properties, they cannot be differentiated on traditional media (PALCAM, Oxford). L. mono Differential Agar Base is based on the formulation of Ottoviani and Agosti (1, 2) for the selective and differential isolation of Listeria monocytogenes from food and animal feeds.
Meat peptone, casein enzymic hydrolysate, yeast extract and sodium pyruvate provide essential growth nutrients and nitrogenous substances. Glucose is the fermentable carbohydrate. Sodium chloride maintains osmotic equilibrium. Phosphate buffers the medium. Lithium chloride and added selective supplements (FD212 and FD213) inhibit accompanying microflora and allow the growth of Listeria species. Listeria species hydrolyse the chromogenic substrate which produces green coloured colonies. Differentiation of Listeria monocytogenes from other Listeria species is based on phosphatidylinositol- specific phospholipase C (PIPLC) activity. Phospholipase C enzyme hydrolyses the purified substrate (FD214) added to the medium resulting in an opaque halo around Listeria monocytogenes colonies.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Please refer disclaimer Overleaf.
HiMedia Laboratories
Technical Data
Colour and Clarity of prepared medium
Light amber coloured, opalescent gel forms in Petri plates
Reaction
Reaction of 7.2% w/v aqueous solution at 25°C. pH : 7.2±0.2
pH
7.00-7.40
Cultural Response
Cultural characteristics observed with added sterile L. mono Selective supplement I (FD212), L. mono Selective Supplement
II (FD213) and L.mono Enrichment supplement I (FD214) after an incubation at 35 - 37°C for 24 - 48 hours.
Cultural Response
Organism
Inoculum
Recovery
Colour of
PIPLC activity
Cultural Response
Candida albicans ATCC
10231Enterococcus faecalis ATCC >=10³ 33090Listeria grayi ATCC 19120 50-100 opaquehalo aroundthe colonyexhibitingphophatidylinositolspecificphospholipaseacivity opaquehalo aroundthe colonyexhibitingphosphatidylinositolspecificphospholipaseactivity Storage and Shelf Life
Store dehydrated powder and the prepared medium at 2-8º C in tightly closed container . Use before expiry date on the label.
Reference
1. Ottaviani F., Ottaviani M., and Agosti M. (1997 a), Industrie Alimentari 36, 1-3.
2.Ottaviani F., Ottaviani M., and Agosti M. (1997 b), Quimper Froid Symposium Proceedings p. 6, A.D.R.I.A. Quimper,
France, 16-18 June 1997.
Please refer disclaimer Overleaf.
HiMedia Laboratories
Technical Data
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notbe considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.
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